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A comparative account of primer designing resources, feature-validations and application-based categorizations

GetPrime

Tool nameGetPrime
URLhttp://updepla1srv1.epfl.ch/getprime/
Important features1. It a gene specific and transcript specific primer database. 2. Database covers 5 species i.e. Homo sapiens (97.5%), Mus musculus (97.9%), Caenorhabditis elegans (57.4%), Drosophila melanogaster (96.6%), Danio rerio (99.3%). 3. It is experimentally and in silico validated database.
CitationsCarine Gubelmann, Alexandre Gattiker, Andreas Massouras, Korneel Hens, Fabrice David, Frederik Decouttere, Jacques Rougemont and Bart Deplancke: GETPrime: a gene- or transcript-specific primer database for quantitative real-time PCR. Database: The Journal of Biological Databases and Curation. 2011 Sep 14;2011: bar040. PubMed PMID: 21917859; PubMed Central PMCID: PMC3173022
Year of publication2011
Rank by usage frequency100
Comments
FunctionJunction primers, Real time PCR, Primer Database
CategoryOnline, Free
Features1. Database holds primer sets that are transcript specific as well as gene specific. 2. Database covers 5 species i.e. Homo sapiens (97.5%), Mus musculus (97.9%), Caenorhabditis elegans (57.4%), Drosophila melanogaster (96.6%), Danio rerio (99.3%). 3. It is experimentally and in silico validated database. 4. It has automated primer specificty checking. User does not have to manually check primer specificty by using BLASTN link.
Description

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